CHARACTERIZATION OF ITS1 SECONDARY STRUCTURE IN TEN SPECIES OF COLUTEOCARPEAE (BRASSICACEAE) AND ITS TAXONOMICAL UTILITY

Utility of the internal transcribed spacers (ITSs) ribosomal RNA sequences to infer phylogenetic relationships among organisms have been proven. Although ITS1 and ITS2 are highly variable in sequence, they conserved structures that a key function processing rRNA gene transcripts. Determining such motif can help identify between organisms. Since has much more secondary structure, structural properties generally neglected by researchers. In this study, ten representative species, which were once assigned under different genera, tribe Coluteocarpeae determined. Also taxonomical utility structure was also tested. Analyses indicate there four types (4-, 6-, 7- 8 hairpin) structures. On other hand, each region is common land plants. previous studies reveals belong tribes or lineages Brassicaceae show similar it not useful delimitation tool for investigated species terms taxonomy.